single polypeptide FPX biosensor for caspase-3
(Plasmid #60883)

• Purpose: FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
• Depositing Lab: Robert Campbell
• Publication: Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.

Backbone

• Vector backbone: pcDNA3 (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7720
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: single polypeptide FPX biosensor for caspase-3
• Alt name: ddRFP A-ddFP B-DEVD-ddGFP A
• Alt name: dimerization dependent red or green fluorescent protein (ddRFP or ddGFP) copy A and copy B
• Species: Synthetic; synthetic construct
• Insert Size (bp): 2220
• GenBank ID: KM891581
• Promoter: CMV
• Tags / Fusion Proteins:
  • NES sequence LALKLAGLDIGS (C terminal on insert)
  • HindIII site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter forward primer
• 3′ sequencing primer: BGH Reverse primers

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry