pcDNA3-CTE-Luc
(Plasmid
#60881)
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PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuc-CTE
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Insert Size (bp)3639
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer 5' taa caa ctc cgc ccc att ga 3’
- 3′ sequencing primer 5’ atg caa ttt cct cat ttt at 3’ (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-CTE-Luc was a gift from Vinay Nandicoori (Addgene plasmid # 60881 ; http://n2t.net/addgene:60881 ; RRID:Addgene_60881) -
For your References section:
Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. Rajanala K, Nandicoori VK. PLoS One. 2012;7(1):e29921. doi: 10.1371/journal.pone.0029921. Epub 2012 Jan 13. 10.1371/journal.pone.0029921 PubMed 22253824
Map uploaded by the depositor.
