GST-PBD
(Plasmid
#60880)
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PurposeBacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-2
- Backbone size w/o insert (bp) 4969
- Total vector size (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePolo-like kinase 1
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Alt namePlk1
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SpeciesH. sapiens (human); BC01486
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Insert Size (bp)712
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GenBank IDNM_005030.3
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Entrez GenePLK1 (a.k.a. PLK, STPK13)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GGG CTG GCA AGC CAC GTT TGG TG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-PBD was a gift from Trina Schroer (Addgene plasmid # 60880 ; http://n2t.net/addgene:60880 ; RRID:Addgene_60880) -
For your References section:
Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. Yeh TY, Kowalska AK, Scipioni BR, Cheong FK, Zheng M, Derewenda U, Derewenda ZS, Schroer TA. EMBO J. 2013 Apr 3;32(7):1023-35. doi: 10.1038/emboj.2013.30. Epub 2013 Mar 1. 10.1038/emboj.2013.30 PubMed 23455152