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GST-PBD H538A/K540M
(Plasmid #60879)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60879 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-2
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Polo-like kinase 1
  • Alt name
    Plk1
  • Species
    H. sapiens (human); BC01486
  • Insert Size (bp)
    712
  • Mutation
    H538A/K540M
  • GenBank ID
    NM_005030.3
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGG CTG GCA AGC CAC GTT TGG TG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PBD H538A/K540M was a gift from Trina Schroer (Addgene plasmid # 60879 ; http://n2t.net/addgene:60879 ; RRID:Addgene_60879)

  • For your References section:

    Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. Yeh TY, Kowalska AK, Scipioni BR, Cheong FK, Zheng M, Derewenda U, Derewenda ZS, Schroer TA. EMBO J. 2013 Apr 3;32(7):1023-35. doi: 10.1038/emboj.2013.30. Epub 2013 Mar 1. 10.1038/emboj.2013.30 PubMed 23455152
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