CIP2Aprom27bp-pGL4.10Luc
(Plasmid #60876)

• Purpose: Luc reporter driven by 27 bp of the CIP2A promoter
• Depositing Lab: Jukka Westermarck
• Publication: Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi: 10.1371/journal.pone.0017979.

Backbone

• Vector backbone: pGL4.10(luc2)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Vector type: Luciferase ; Promoterless

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 27 bp promoter fragment of Cancerous Inhibitor of PP2A
• Alt name: 27 bp CIP2A promoter
• Species: H. sapiens (human)
• Insert Size (bp): 27
• Entrez Gene: CIP2A (a.k.a. KIAA1524, NOCIVA, p90)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Then various length luciferase promoter constructs were created using the Deletion Kit for Kilo-Sequencing (Takara Bio Inc., Japan) as per the manufacturer’s instructions. Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.