CIP2Aprom865bp-pGL4.10Luc
(Plasmid
#60869)
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PurposeLuc reporter driven by 865 bp of the CIP2A promoter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.10(luc2)
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4242
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Vector typeLuciferase ; Promoterless
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name865 bp promoter fragment of Cancerous Inhibitor of PP2A
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Alt name865 bp CIP2A promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)865
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Entrez GeneCIP2A (a.k.a. KIAA1524, NOCIVA, p90)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Then various length luciferase promoter constructs were created using the Deletion Kit for Kilo-Sequencing (Takara Bio Inc., Japan) as per the manufacturer’s instructions. Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CIP2Aprom865bp-pGL4.10Luc was a gift from Jukka Westermarck (Addgene plasmid # 60869 ; http://n2t.net/addgene:60869 ; RRID:Addgene_60869) -
For your References section:
ETS1 mediates MEK1/2-dependent overexpression of cancerous inhibitor of protein phosphatase 2A (CIP2A) in human cancer cells. Khanna A, Okkeri J, Bilgen T, Tiirikka T, Vihinen M, Visakorpi T, Westermarck J. PLoS One. 2011 Mar 22;6(3):e17979. doi: 10.1371/journal.pone.0017979. 10.1371/journal.pone.0017979 PubMed 21445343
Map uploaded by the depositor.