pCAS Citations (20)
Originally described in: Selection of chromosomal DNA libraries using a multiplex CRISPR system.Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, DeLoache W, Dueber JE, Arkin AP, Cate JH Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703. PubMed Journal
Articles Citing pCAS
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| CRISPR-Cas9 Genome Engineering in Saccharomyces cerevisiae Cells. Ryan OW, Poddar S, Cate JH. Cold Spring Harb Protoc. 2016 Jun 1;2016(6):pdb.prot086827. doi: 10.1101/pdb.prot086827. PubMed |
| Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines. McKeague M, Wang YH, Cravens A, Win MN, Smolke CD. Metab Eng. 2016 Nov;38:191-203. doi: 10.1016/j.ymben.2016.08.003. Epub 2016 Aug 9. PubMed |
| Marker-free genetic manipulations in yeast using CRISPR/CAS9 system. Soreanu I, Hendler A, Dahan D, Dovrat D, Aharoni A. Curr Genet. 2018 Oct;64(5):1129-1139. doi: 10.1007/s00294-018-0831-y. Epub 2018 Apr 6. PubMed |
| A Live-Cell Imaging Approach for Measuring DNA Replication Rates. Dovrat D, Dahan D, Sherman S, Tsirkas I, Elia N, Aharoni A. Cell Rep. 2018 Jul 3;24(1):252-258. doi: 10.1016/j.celrep.2018.06.018. PubMed |
| CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory Course. Sehgal N, Sylves ME, Sahoo A, Chow J, Walker SE, Cullen PJ, Berry JO. Biochem Mol Biol Educ. 2018 Nov;46(6):592-601. doi: 10.1002/bmb.21175. Epub 2018 Oct 12. PubMed |
| The Chaperonin TRiC/CCT Associates with Prefoldin through a Conserved Electrostatic Interface Essential for Cellular Proteostasis. Gestaut D, Roh SH, Ma B, Pintilie G, Joachimiak LA, Leitner A, Walzthoeni T, Aebersold R, Chiu W, Frydman J. Cell. 2019 Apr 18;177(3):751-765.e15. doi: 10.1016/j.cell.2019.03.012. Epub 2019 Apr 4. PubMed |
| The Magnitude of IFN-gamma Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1. Petermann F, Pekowska A, Johnson CA, Jankovic D, Shih HY, Jiang K, Hudson WH, Brooks SR, Sun HW, Villarino AV, Yao C, Singleton K, Akondy RS, Kanno Y, Sher A, Casellas R, Ahmed R, O'Shea JJ. Mol Cell. 2019 Sep 19;75(6):1229-1242.e5. doi: 10.1016/j.molcel.2019.06.025. Epub 2019 Jul 31. PubMed |
| A conserved Bcd1 interaction essential for box C/D snoRNP biogenesis. Khoshnevis S, Dreggors RE, Hoffmann TFR, Ghalei H. J Biol Chem. 2019 Nov 29;294(48):18360-18371. doi: 10.1074/jbc.RA119.010222. Epub 2019 Sep 19. PubMed |
| Intragenic suppressor mutations of the COQ8 protein kinase homolog restore coenzyme Q biosynthesis and function in Saccharomyces cerevisiae. Awad AM, Nag A, Pham NVB, Bradley MC, Jabassini N, Nathaniel J, Clarke CF. PLoS One. 2020 Jun 1;15(6):e0234192. doi: 10.1371/journal.pone.0234192. eCollection 2020. PubMed |
Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering.
Cravens A, Jamil OK, Kong D, Sockolosky JT, Smolke CD.
Nat Commun. 2021 Mar 11;12(1):1579. doi: 10.1038/s41467-021-21876-z.
PubMed
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| Protein context shapes the specificity of SH3 domain-mediated interactions in vivo. Dionne U, Bourgault E, Dube AK, Bradley D, Chartier FJM, Dandage R, Dibyachintan S, Despres PC, Gish GD, Pham NTH, Letourneau M, Lambert JP, Doucet N, Bisson N, Landry CR. Nat Commun. 2021 Mar 12;12(1):1597. doi: 10.1038/s41467-021-21873-2. PubMed |
| Stress tolerance enhancement via SPT15 base editing in Saccharomyces cerevisiae. Liu Y, Lin Y, Guo Y, Wu F, Zhang Y, Qi X, Wang Z, Wang Q. Biotechnol Biofuels. 2021 Jul 6;14(1):155. doi: 10.1186/s13068-021-02005-w. PubMed |
| Efficient Conversion of Glycerol to Ethanol by an Engineered Saccharomyces cerevisiae Strain. Khattab SMR, Watanabe T. Appl Environ Microbiol. 2021 Nov 10;87(23):e0026821. doi: 10.1128/AEM.00268-21. Epub 2021 Sep 15. PubMed |
| Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor. Wang J, Teng Y, Zhang R, Wu Y, Lou L, Zou Y, Li M, Xie ZR, Yan Y. Nat Commun. 2021 Nov 25;12(1):6916. doi: 10.1038/s41467-021-27290-9. PubMed |
| Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens. Armaleo D, Chiou L. G3 (Bethesda). 2021 Oct 19;11(11). pii: 6347584. doi: 10.1093/g3journal/jkab279. PubMed |
The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit.
Bean BDM, Whiteway M, Martin VJJ.
G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 10.1093/g3journal/jkac154.
PubMed
Associated Plasmids |
| A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae. Kim MJ, Sung BH, Park HJ, Sohn JH, Bae JH. Biotechnol Rep (Amst). 2022 Sep 11;36:e00763. doi: 10.1016/j.btre.2022.e00763. eCollection 2022 Dec. PubMed |
| Sequence and chromatin features guide DNA double-strand break resection initiation. Gnugge R, Reginato G, Cejka P, Symington LS. Mol Cell. 2023 Apr 20;83(8):1237-1250.e15. doi: 10.1016/j.molcel.2023.02.010. Epub 2023 Mar 13. PubMed |
| Direct and indirect consequences of PAB1 deletion in the regulation of translation initiation, translation termination, and mRNA decay. Mangkalaphiban K, Ganesan R, Jacobson A. bioRxiv [Preprint]. 2023 Jun 1:2023.05.31.543082. doi: 10.1101/2023.05.31.543082. PubMed |
| Mechanism of assembly of snRNP cores assisted by ICln and the SMN complex in fission yeast. Hu Y, Hou Y, Zhou S, Wang Y, Shen C, Mu L, Su D, Zhang R. iScience. 2023 Aug 12;26(9):107604. doi: 10.1016/j.isci.2023.107604. eCollection 2023 Sep 15. PubMed |
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