pTargeT gE
(Plasmid #60845)

• Purpose: Expression of the varicella zoster glycoprotein (gE) in mammalian cells
• Depositing Lab: Charles Grose
• Publication: Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 10.1128/JVI.00281-11. Epub 2011 Jul 13.

Backbone

• Vector backbone: pTargeT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ORF68
• Alt name: VZV gE
• Alt name: varicella glycoprotein E
• Insert Size (bp): 1869
• GenBank ID: NC_001348.1
• Entrez Gene: ORF68 (a.k.a. HHV3_gp69)
• Promoter: CMV

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: N/A
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry