AAVS1-Neo-M2rtTA
(Plasmid #60843)

• Purpose: AAVS1 donor vector for genomic targeting
• Depositing Lab: Rudolf Jaenisch
• Publication: DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi: 10.1101/gr.106773.110. Epub 2010 May 27.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 8516
• Total vector size (bp): 10098
• Vector type: targeting donor
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: M2rtTA
• Insert Size (bp): 782

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F

Gene/Insert 2

• Gene/Insert name: Neo
• Insert Size (bp): 800

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: M13rev

Resource Information

• Supplemental Documents:
  • GenBank file
• Addgene Notes:
  • Addgene Diagnostic Test
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: M2-rtTA was obtained from Dr. H. Bujard
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry