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pET15b-ITPA_P32T
(Plasmid #60825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    inosine triphosphate pyrophosphohydrolase
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ITPA
  • Alt name
    inosine triphosphate pyrophosphohydrolase
  • Species
    H. sapiens (human)
  • Mutation
    P32T
  • Entrez Gene
    ITPA (a.k.a. C20orf37, DEE35, HLC14-06-P, ITPase, My049, NTPase, dJ794I6.3)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-ITPA_P32T was a gift from Youri Pavlov (Addgene plasmid # 60825 ; http://n2t.net/addgene:60825 ; RRID:Addgene_60825)

  • For your References section:

    Functional study of the P32T ITPA variant associated with drug sensitivity in humans. Stepchenkova EI, Tarakhovskaya ER, Spitler K, Frahm C, Menezes MR, Simone PD, Kolar C, Marky LA, Borgstahl GE, Pavlov YI. J Mol Biol. 2009 Sep 25;392(3):602-13. doi: 10.1016/j.jmb.2009.07.051. Epub 2009 Jul 23. 10.1016/j.jmb.2009.07.051 PubMed 19631656
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