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pET15b-ITPA
(Plasmid #60824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    inosine triphosphate pyrophosphohydrolase
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ITPA
  • Alt name
    inosine triphosphate pyrophosphohydrolase
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITPA (a.k.a. C20orf37, DEE35, HLC14-06-P, ITPase, My049, NTPase, dJ794I6.3)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dr. Stas Kozmin (NIEHS) contributed to the creation of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-ITPA was a gift from Youri Pavlov (Addgene plasmid # 60824 ; http://n2t.net/addgene:60824 ; RRID:Addgene_60824)

  • For your References section:

    Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase. Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1076-81. Epub 2006 Oct 25. 10.1107/S1744309106041790 PubMed 17077483
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