pESC-LEU2-RAD30cmyc
(Plasmid
#60821)
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Purposeto overexpress yeast polymerase eta in yeast
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepESC-LEU2
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRAD30
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Alt nameDNA Polymerase eta
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneRAD30 (a.k.a. YDR419W, DBH1)
- Promoter GAL1
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Tag
/ Fusion Protein
- c-myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer Gal1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pESC-LEU2-RAD30cmyc was a gift from Thomas Kunkel & Youri Pavlov (Addgene plasmid # 60821 ; http://n2t.net/addgene:60821 ; RRID:Addgene_60821) -
For your References section:
Mutator effects of overproducing DNA polymerase eta (Rad30) and its catalytically inactive variant in yeast. Pavlov YI, Nguyen D, Kunkel TA. Mutat Res. 2001 Jul 1;478(1-2):129-39. S0027510701001312 [pii] PubMed 11406177