pESC-URA-hPOLISc
(Plasmid
#60819)
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Purposeto express human Pol iota recoded to yeast codons in yeast
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepESC-URA
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePOLI
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Alt nameDNA polymerase iota
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SpeciesH. sapiens (human)
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Mutationoptimized to yeast codon usage
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Entrez GenePOLI (a.k.a. RAD30B, RAD3OB, eta2)
- Promoter GAL1
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Tag
/ Fusion Protein
- c-myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer Gal1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pESC-URA-hPOLISc was a gift from Youri Pavlov (Addgene plasmid # 60819 ; http://n2t.net/addgene:60819 ; RRID:Addgene_60819) -
For your References section:
Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota. Makarova AV, Grabow C, Gening LV, Tarantul VZ, Tahirov TH, Bessho T, Pavlov YI. PLoS One. 2011 Jan 31;6(1):e16612. doi: 10.1371/journal.pone.0016612. 10.1371/journal.pone.0016612 PubMed 21304950
