Skip to main content
Addgene

pESC-URA-hPOLISc
(Plasmid #60819)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pESC-URA
  • Backbone manufacturer
    Stratagene
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    POLI
  • Alt name
    DNA polymerase iota
  • Species
    H. sapiens (human)
  • Mutation
    optimized to yeast codon usage
  • Entrez Gene
    POLI (a.k.a. RAD30B, RAD3OB, eta2)
  • Promoter GAL1
  • Tag / Fusion Protein
    • c-myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer Gal1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESC-URA-hPOLISc was a gift from Youri Pavlov (Addgene plasmid # 60819 ; http://n2t.net/addgene:60819 ; RRID:Addgene_60819)

  • For your References section:

    Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota. Makarova AV, Grabow C, Gening LV, Tarantul VZ, Tahirov TH, Bessho T, Pavlov YI. PLoS One. 2011 Jan 31;6(1):e16612. doi: 10.1371/journal.pone.0016612. 10.1371/journal.pone.0016612 PubMed 21304950
Related items:
Commonly requested with: