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pESC-LEU-PmCDA1
(Plasmid #60818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESC-LEU
  • Backbone manufacturer
    Stratagene
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDA1
  • Alt name
    PmCDA.21 cytosine deaminase
  • Species
    sea lamprey (Petromyzon marinus)
  • GenBank ID
    EF094822
  • Promoter GAL10
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer Gal10Pro_F_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Masayuki Hirano (Emory University) and Igor Rogozin (NCBI) contributed to the creation of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESC-LEU-PmCDA1 was a gift from Youri Pavlov (Addgene plasmid # 60818 ; http://n2t.net/addgene:60818 ; RRID:Addgene_60818)

  • For your References section:

    Genome-wide mutation avalanches induced in diploid yeast cells by a base analog or an APOBEC deaminase. Lada AG, Stepchenkova EI, Waisertreiger IS, Noskov VN, Dhar A, Eudy JD, Boissy RJ, Hirano M, Rogozin IB, Pavlov YI. PLoS Genet. 2013;9(9):e1003736. doi: 10.1371/journal.pgen.1003736. Epub 2013 Sep 5. PGENETICS-D-13-01361 [pii] PubMed 24039593
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