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PurposedCas9 with single cysteine residue (M1C) for site-specific labelling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHMGWA
- Total vector size (bp) 10076
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Modifications to backboneadditional of 4 His to 6X His for higher Ni-NTA affinity
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9 M1C D10A C80S H840A C574S
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SpeciesS. pyogenes
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MutationM1C D10A C80S H840A C574S
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Tags
/ Fusion Proteins
- His10 (N terminal on insert)
- MBP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MBP (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S was a gift from Jennifer Doudna (Addgene plasmid # 60815 ; http://n2t.net/addgene:60815 ; RRID:Addgene_60815) -
For your References section:
Programmable RNA recognition and cleavage by CRISPR/Cas9. O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, Doudna JA. Nature. 2014 Sep 28. doi: 10.1038/nature13769. 10.1038/nature13769 PubMed 25274302