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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT
(Plasmid #60814)

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Item Catalog # Description Quantity Price (USD)
Plasmid 60814 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA-FRT/T0-eGFPnls
  • Backbone size w/o insert (bp) 5158
  • Total vector size (bp) 6391
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1233
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Tag / Fusion Protein
    • eGFP-NLS (SV40) (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT was a gift from Daniel Durocher (Addgene plasmid # 60814 ; http://n2t.net/addgene:60814 ; RRID:Addgene_60814)

  • For your References section:

    53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. 10.1038/nature12318 PubMed 23760478
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