Skip to main content
Addgene

pRevLCav2deltaC
(Plasmid #60809)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pYIY118
  • Backbone manufacturer
    this lab
  • Modifications to backbone
    URA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    REV3
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    rev3ΔC encodes for Rev3 lacking the C-terminus (amino acids 1381–1504)
  • Entrez Gene
    REV3 (a.k.a. YPL167C, PSO1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Artem Lada in the Pavlov lab and Tahir Tahirov (UNMC) contributed to the creation of this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRevLCav2deltaC was a gift from Youri Pavlov & Tahir Tahirov (Addgene plasmid # 60809 ; http://n2t.net/addgene:60809 ; RRID:Addgene_60809)

  • For your References section:

    A novel variant of DNA polymerase zeta, Rev3DeltaC, highlights differential regulation of Pol32 as a subunit of polymerase delta versus zeta in Saccharomyces cerevisiae. Siebler HM, Lada AG, Baranovskiy AG, Tahirov TH, Pavlov YI. DNA Repair (Amst). 2014 May 9. pii: S1568-7864(14)00135-9. doi: 10.1016/j.dnarep.2014.04.013. 10.1016/j.dnarep.2014.04.013 PubMed 24819597
Related items: