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pRevLCav2FeS
(Plasmid #60808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYIY118
  • Backbone manufacturer
    this lab
  • Modifications to backbone
    URA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    REV3
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    contains 647 C-terminal amino acids and 335 bp of downstream noncoding sequence, change of cysteines 1446, 1449, 1468, and 1473 at MBS2 to alanines
  • Entrez Gene
    REV3 (a.k.a. YPL167C, PSO1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Artem Lada in the Pavlov lab and Tahir Tahirov (UNMC) contributed to the creation of this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRevLCav2FeS was a gift from Youri Pavlov & Tahir Tahirov (Addgene plasmid # 60808 ; http://n2t.net/addgene:60808 ; RRID:Addgene_60808)

  • For your References section:

    DNA polymerase delta and zeta switch by sharing accessory subunits of DNA polymerase delta. Baranovskiy AG, Lada AG, Siebler HM, Zhang Y, Pavlov YI, Tahirov TH. J Biol Chem. 2012 May 18;287(21):17281-7. doi: 10.1074/jbc.M112.351122. Epub 2012 Mar 30. 10.1074/jbc.M112.351122 PubMed 22465957
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