pYIAL31
(Plasmid
#60807)
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Purposeused to make mutations in yeast POL1
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYIY118
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Backbone manufacturerthis lab
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Modifications to backboneURA3 gene cloned into BstZ17-I site of the low copy number plasmid pMBL18.
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePol1
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Alt nameDNA-directed DNA polymerase alpha catalytic subunit POL1
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SpeciesS. cerevisiae (budding yeast)
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MutationN-terminal part only
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Entrez GenePOL1 (a.k.a. YNL102W, CDC17, CRT5, HPR3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYIAL31 was a gift from Thomas Kunkel & Youri Pavlov (Addgene plasmid # 60807 ; http://n2t.net/addgene:60807 ; RRID:Addgene_60807) -
For your References section:
In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta. Pavlov YI, Shcherbakova PV, Kunkel TA. Genetics. 2001 Sep;159(1):47-64. PubMed 11560886
Map uploaded by the depositor.
