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Addgene

pIS1 mHK1
(Plasmid #60795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIS1
  • Backbone manufacturer
    Bartel lab (Addgene plasmid # 12179)
  • Backbone size w/o insert (bp) 4085
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HK1 3'UTR and mutated miR-155 binding site
  • Species
    H. sapiens (human)
  • Mutation
    mutated miR-155 binding site
  • Entrez Gene
    HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase)
  • Promoter thymidine kinase (HSV-TK promoter)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3') to confirm luciferase
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIS1 mHK1 was a gift from David Bartel (Addgene plasmid # 60795 ; http://n2t.net/addgene:60795 ; RRID:Addgene_60795)

  • For your References section:

    Global analyses of the effect of different cellular contexts on microRNA targeting. Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal V, Yildirim MA, Rodriguez A, Bartel DP. Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13. 10.1016/j.molcel.2014.02.013 PubMed 24631284
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