pIS1 mELOVL6
(Plasmid #60791)

• Purpose: Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and mutated miR-155 sites
• Depositing Lab: David Bartel
• Publication: Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.

Backbone

• Vector backbone: pIS1
• Backbone manufacturer: Bartel lab (Addgene plasmid # 12179)
• Backbone size w/o insert (bp): 4085
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ELOVL6 3'UTR and mutated miR-155 binding site
• Species: H. sapiens (human)
• Mutation: mutated miR-155 binding site
• Entrez Gene: ELOVL6 (a.k.a. FACE, FAE, LCE)
• Promoter: thymidine kinase (HSV-TK promoter)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3') to confirm luciferase
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • pIS1 mELOVL6 annotated GenBank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry