pTriEx-mCherry::LANS1
(Plasmid #60784)

• Purpose: Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation
• Depositing Lab: Brian Kuhlman
• Publication: Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. doi: 10.1371/journal.pone.0128443. eCollection 2015.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5943
• Total vector size (bp): 6429
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LANS1
• Species: Synthetic; Avena Sativa
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CCCAACACAATATATTATAGTTAAATAAGAATTATTATC
• 3′ sequencing primer: GGTGGTGCTCGAGATCCTCGGGG

Resource Information

• Supplemental Documents:
  • LANS1
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry