pFA6a-link-GFPEnvy-SpHis5
(Plasmid #60782)

• Purpose: Yeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selection
• Depositing Lab: Linda Huang
• Publication: Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.3065.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4021
• Total vector size (bp): 4738
• Vector type: Yeast Integrating
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFPEnvy
• Alt name: GreenFluorescentProtein Envy
• Species: Synthetic; A. victoria
• Insert Size (bp): 717
• Mutation: S30R Y39N T65C S72A F99S N105T Y145F M153T V163A I167T I171V A206V
• GenBank ID: KM891731

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: pHybLex_rev_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene 44837
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry