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pZA2[PLac:mCherry,PTAN:GFP]
(Plasmid #60780)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60780 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Backbone size w/o insert (bp) 3532
  • Total vector size (bp) 4957
  • Modifications to backbone
    Contains one Plac driven ORF for the regulated expression of mCherry. Also contains one PTAN driven ORF for the regulated expression of yemGFP.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    708

Gene/Insert 2

  • Gene/Insert name
    yemGFP
  • Alt name
    Yeast Enhanced GFP
  • Insert Size (bp)
    717

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancies between full and QC sequences have no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZA2[PLac:mCherry,PTAN:GFP] was a gift from Matthew Bennett (Addgene plasmid # 60780 ; http://n2t.net/addgene:60780 ; RRID:Addgene_60780)

  • For your References section:

    Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28. 10.1021/sb500262f PubMed 25035932
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