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pZM3[I:FruR-L,I:RbsR-L]
(Plasmid #60777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-b
  • Backbone size w/o insert (bp) 3366
  • Total vector size (bp) 2289
  • Modifications to backbone
    Heavily modified pET28b backbone. Deletion of lacI ORF and T7 ORF except for T7 terminator. Antibiotic resistance changed from KanR to CmR.

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FruR-L
  • Alt name
    LLhF_Q60S
  • Species
    Synthetic
  • Insert Size (bp)
    1005
  • Mutation
    LacI/GalR repressor chimera. LacI DBD with FruR LBD. Contains point mutation Q60S

Gene/Insert 2

  • Gene/Insert name
    RbsR-L
  • Alt name
    LLhR_Q60A
  • Species
    Synthetic
  • Insert Size (bp)
    1002
  • Mutation
    LacI/GalR repressor chimera. LacI DBD with RbsR LBD. Contains point mutation Q60A

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FruR-L and RbsR-L also described as LLhF_Q60S and LLhR_Q60A respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZM3[I:FruR-L,I:RbsR-L] was a gift from Matthew Bennett (Addgene plasmid # 60777 ; http://n2t.net/addgene:60777 ; RRID:Addgene_60777)

  • For your References section:

    Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28. 10.1021/sb500262f PubMed 25035932
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