pZS1[RbsR-T,TreR-T]
(Plasmid #60776)

• Purpose: Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.
• Depositing Lab: Matthew Bennett
• Publication: Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.

Backbone

• Vector backbone: pZS5
• Backbone manufacturer: Expressys
• Backbone size w/o insert (bp): 4040
• Total vector size (bp): 6188
• Modifications to backbone: Insertion of a PIq driven orf for expression of RbsR-T and a PI driven ORF for expression of TreR-T.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: RbsR-L
• Alt name: LLhR_Q60A, Y17T, Q18A, R22N
• Species: Synthetic
• Insert Size (bp): 999
• Mutation: LacI/GalR repressor chimera. TAN DBD with RbsR LBD. Contains point mutation Q60A

Gene/Insert 2

• Gene/Insert name: TreR-T
• Alt name: LLHT_V52A, Y17T, Q18A, R22N
• Species: Synthetic
• Insert Size (bp): 945
• Mutation: LacI/GalR repressor chimera. TAN DBD with TreR LBD. Contains point mutation V52A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry