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pZS1[GalS-L,TreR-L]
(Plasmid #60765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZS5
  • Backbone manufacturer
    Expressys
  • Backbone size w/o insert (bp) 4040
  • Total vector size (bp) 6236
  • Modifications to backbone
    Insertion of PIq driven ORF, for GalS-L, and one PI driven ORF, for TreR-L.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    GalS-L
  • Alt name
    LLhS_Q54A
  • Species
    Synthetic
  • Insert Size (bp)
    1048
  • Mutation
    LacI/GalR repressor chimera. LacI DBD with GalS LBD. Contains point mutation Q54A, E191K

Gene/Insert 2

  • Gene/Insert name
    TreR-L
  • Alt name
    LLhT_V52A
  • Species
    Synthetic
  • Insert Size (bp)
    945
  • Mutation
    LacI/GalR repressor chimera. LacI DBD with TreR LBD. Contains point mutation V52A

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GalS-L and TreR-L also described as LLhS_Q54A and TreR_V52A respectively in
PLoS One. 2013 Dec 30;8(12):e83502. doi: 10.1371/journal.pone.0083502. eCollection 2013.
Rheostats and toggle switches for modulating protein function.
Meinhardt S, Manley MW Jr, Parente DJ, Swint-Kruse L.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS1[GalS-L,TreR-L] was a gift from Matthew Bennett (Addgene plasmid # 60765 ; http://n2t.net/addgene:60765 ; RRID:Addgene_60765)

  • For your References section:

    Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28. 10.1021/sb500262f PubMed 25035932
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