pZS1[FruR-T]
(Plasmid #60752)

• Purpose: Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
• Depositing Lab: Matthew Bennett
• Publication: Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.

Backbone

• Vector backbone: pZS5
• Backbone manufacturer: Expressys
• Backbone size w/o insert (bp): 4040
• Total vector size (bp): 5045
• Modifications to backbone: Insertion of a PIq driven ORF.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: FruR-T
• Alt name: LLhF_Q60S, Y17T, Q18A, R22N
• Species: E. Coli
• Insert Size (bp): 1005
• Mutation: Chimeric LacI/GalR repressor with the TAN DBD and the FruR LBD.

Gene/Insert 2

• Gene/Insert name: FruR-T
• Alt name: LLhF_Q60S
• Alt name: Y17T, Q18A, R22N
• Species: E. Coli
• Insert Size (bp): 1005
• Mutation: Chimeric LacI/GalR repressor with the TAN DBD and the RbsR LBD.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry