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Addgene

pZS1[RbsR-T]
(Plasmid #60751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZS5
  • Backbone manufacturer
    Expressys
  • Backbone size w/o insert (bp) 4040
  • Total vector size (bp) 5039
  • Modifications to backbone
    Insertion of a PIq driven ORF.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RbsR-T
  • Alt name
    LLhR_Q60A, Y17T, Q18A, R22N
  • Species
    E. Coli
  • Insert Size (bp)
    999
  • Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and the RbsR LBD.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS1[RbsR-T] was a gift from Matthew Bennett (Addgene plasmid # 60751 ; http://n2t.net/addgene:60751 ; RRID:Addgene_60751)

  • For your References section:

    Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28. 10.1021/sb500262f PubMed 25035932
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