bfloGFPa1
(Plasmid #60749)

• Purpose: Bacterial expression of Amphioxus GFPa1
• Depositing Lab: Dimitri Deheyn
• Publication: Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.

Backbone

• Vector backbone: pET24b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Protein can be expressed in BL21 codon+ cells. It should be grown at 37C for 4 hours followed by 20C for 6 hours.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFPa1
• Species: Branchiostoma floridae (amphioxus)
• Insert Size (bp): 741
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-Term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry