pTSara-R756S
(Plasmid #60725)

• Purpose: Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
• Depositing Lab: Matthew Bennett
• Publication: Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.

Backbone

• Vector backbone: pBAD33
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7600
• Modifications to backbone: IPTG inducible promoter PLac drives expression 1-179 of split T7 RNAP while the arabinose promoter PBAD drives expression 180-880.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Residues 1-179 of split T7 RNAP
• Species: Synthetic; T7 Phage
• Insert Size (bp): 540
• Mutation: Residues 1-180 of split T7 RNAP
• GenBank ID: GI:216012

Gene/Insert 2

• Gene/Insert name: Residues 180-880 of split T7 RNAP
• Species: Synthetic
• Insert Size (bp): 2118
• Mutation: Residues 180-880 of split T7 RNAP. Contains mutation R756S
• GenBank ID: GI:216012

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry