pcDNA-Cx43-YA/VD
(Plasmid
#60699)
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Purposemammalian expression of rat Cx43 YA/VD mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCx43
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Alt namegap junction protein, alpha 1
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Alt nameconnexin43
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SpeciesR. norvegicus (rat)
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MutationYA/VD (Y286A/V289D)
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Entrez GeneGja1 (a.k.a. Cx43, Cxnk1)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Cx43-YA/VD was a gift from Parmender Mehta (Addgene plasmid # 60699 ; http://n2t.net/addgene:60699 ; RRID:Addgene_60699) -
For your References section:
Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells. Johnson KE, Mitra S, Katoch P, Kelsey LS, Johnson KR, Mehta PP. Mol Biol Cell. 2013 Mar;24(6):715-33. doi: 10.1091/mbc.E12-07-0537. Epub 2013 Jan 30. 10.1091/mbc.E12-07-0537 PubMed 23363606