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Addgene

LXSN-Cx43-mAPPLE
(Plasmid #60696)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLXSN
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cx43
  • Alt name
    gap junction protein, alpha 1
  • Alt name
    connexin43
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gja1 (a.k.a. Cx43, Cxnk1)
  • Promoter LTR
  • Tag / Fusion Protein
    • mAPPLE (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LXSN-Cx43-mAPPLE was a gift from Parmender Mehta (Addgene plasmid # 60696 ; http://n2t.net/addgene:60696 ; RRID:Addgene_60696)

  • For your References section:

    Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells. Johnson KE, Mitra S, Katoch P, Kelsey LS, Johnson KR, Mehta PP. Mol Biol Cell. 2013 Mar;24(6):715-33. doi: 10.1091/mbc.E12-07-0537. Epub 2013 Jan 30. 10.1091/mbc.E12-07-0537 PubMed 23363606
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