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Addgene

SpDJ-1 pET15b
(Plasmid #60689)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DJ-1
  • Alt name
    SPAC22E12.03c
  • Alt name
    (See Comments)
  • Species
    S. pombe (fission yeast)
  • Entrez Gene
    SPAC22E12.03c (a.k.a. SPAC22E12.03c)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence is synthetic with codon usage optimized for E.coli expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpDJ-1 pET15b was a gift from Mark Wilson (Addgene plasmid # 60689 ; http://n2t.net/addgene:60689 ; RRID:Addgene_60689)

  • For your References section:

    Influence of peptide dipoles and hydrogen bonds on reactive cysteine pKa values in fission yeast DJ-1. Madzelan P, Labunska T, Wilson MA. FEBS J. 2012 Nov;279(22):4111-20. doi: 10.1111/febs.12004. Epub 2012 Oct 11. 10.1111/febs.12004 PubMed 22971103
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