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Addgene

PfICH pET15b
(Plasmid #60684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    inhA
  • Alt name
    Isocyanide Hydratase
  • Species
    Pseudomonas fluorescens
  • GenBank ID
    NC_016830.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PfICH pET15b was a gift from Mark Wilson (Addgene plasmid # 60684 ; http://n2t.net/addgene:60684 ; RRID:Addgene_60684)

  • For your References section:

    Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase. Lakshminarasimhan M, Madzelan P, Nan R, Milkovic NM, Wilson MA. J Biol Chem. 2010 Sep 17;285(38):29651-61. doi: 10.1074/jbc.M110.147934. Epub 2010 Jul 14. 10.1074/jbc.M110.147934 PubMed 20630867
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