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Addgene

M26I DJ-1 pET15b
(Plasmid #60677)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M26I DJ-1
  • Alt name
    Park7
  • Species
    H. sapiens (human)
  • Mutation
    M26I
  • Entrez Gene
    PARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M26I DJ-1 pET15b was a gift from Mark Wilson (Addgene plasmid # 60677 ; http://n2t.net/addgene:60677 ; RRID:Addgene_60677)

  • For your References section:

    Structural impact of three Parkinsonism-associated missense mutations on human DJ-1. Lakshminarasimhan M, Maldonado MT, Zhou W, Fink AL, Wilson MA. Biochemistry. 2008 Feb 5;47(5):1381-92. doi: 10.1021/bi701189c. Epub 2008 Jan 9. 10.1021/bi701189c PubMed 18181649
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