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Addgene

pBUN82
(Plasmid #60675)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV262
  • Backbone manufacturer
    MedImmune Inc.
  • Total vector size (bp) 6400
  • Vector type
    E.coli-Mycobacterium shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alrA
  • Alt name
    D-alanine racemase gene
  • Species
    M. smegmatis
  • GenBank ID
    U70872
  • Promoter hsp60

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ScaI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBUN82 was a gift from Raul Barletta (Addgene plasmid # 60675 ; http://n2t.net/addgene:60675 ; RRID:Addgene_60675)

  • For your References section:

    Overexpression of the D-alanine racemase gene confers resistance to D-cycloserine in Mycobacterium smegmatis. Caceres NE, Harris NB, Wellehan JF, Feng Z, Kapur V, Barletta RG. J Bacteriol. 1997 Aug;179(16):5046-55. PubMed 9260945
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