Skip to main content
Addgene

PB-Mycn
(Plasmid #60667)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBTO-Dest
  • Backbone manufacturer
    Thomson lab
  • Backbone size w/o insert (bp) 4182
  • Total vector size (bp) 5571
  • Vector type
    Mammalian Expression ; PiggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mycn
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1389
  • Entrez Gene
    Mycn (a.k.a. N-myc, Nmyc, Nmyc-1, Nmyc1, bHLHe37, c-nmyc)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATCCACGCTGTTTTGACCTC
  • 3′ sequencing primer GCTCTAGAGTCGGTGACTAGTATCAAT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genecopoeia

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-Mycn was a gift from James Thomson (Addgene plasmid # 60667 ; http://n2t.net/addgene:60667 ; RRID:Addgene_60667)

  • For your References section:

    An expandable, inducible hemangioblast state regulated by fibroblast growth factor. Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. 10.1016/j.stemcr.2014.10.003 PubMed 25458896
Related items:
Commonly requested with: