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PurposeFlp-dependent expression of mNaChBac-T2A-tdTomato
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5639
- Total vector size (bp) 7939
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLEX-mNaChBac-T2A-tdTomato
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Speciese. coli
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Insert Size (bp)2300
- Promoter EF1α
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF1a-Fwd
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato was a gift from Massimo Scanziani (Addgene plasmid # 60658 ; http://n2t.net/addgene:60658 ; RRID:Addgene_60658) -
For your References section:
Equalizing excitation-inhibition ratios across visual cortical neurons. Xue M, Atallah BV, Scanziani M. Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. Epub 2014 Jun 22. 10.1038/nature13321 PubMed 25043046