pcDNA 3.1 (+) FLAG-Ago2 S387E
(Plasmid #60653)

• Purpose: Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells
• Depositing Lab: Carl Novina
• Publication: Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Argonaute 2
• Alt name: Protein Slicer
• Alt name: EIF2C 2
• Species: H. sapiens (human)
• Mutation: S387E mutant
• Entrez Gene: AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
• Promoter: CMV
• Tag / Fusion Protein:
  • CMV-FLAG-Ago2 (N-term tag) (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Ago2-S387E-Flag-pcDNA3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation. +/- refers to orientation of f1 ori.