pcDNA3.1(+) CircRNA Mini Vector
(Plasmid #60648)

• Purpose: Expression plasmid for expressing circular RNAs of a desired sequence
• Depositing Lab: Jeremy Wilusz
• Publication: Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.114.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5607
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZKSCAN1
• Species: H. sapiens (human)
• Insert Size (bp): 199
• Entrez Gene: ZKSCAN1 (a.k.a. KOX18, PHZ-37, ZNF139, ZNF36, ZSCAN33)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: PCMV For
• 3′ sequencing primer: bGH PolyA Rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 27 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 51-nt artificial exon composed of restriction enzyme sites was inserted between the minimal introns that support ZKSCAN1 circular RNA production (as described in Figure 7A of Liang and Wilusz (2014) Genes Dev).