pcDNA3.1(+) EPHB4 Antisense
(Plasmid #60640)

• Purpose: Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid
• Depositing Lab: Jeremy Wilusz
• Publication: Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.114.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6876
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ephrin type B receptor 4
• Species: H. sapiens (human)
• Insert Size (bp): 1428
• Entrez Gene: EPHB4 (a.k.a. CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: PCMV For
• 3′ sequencing primer: bGH PolyA Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is the same as "pcDNA3.1(+) EPHB4 Sense" except that the 1428-nt insert is in the antisense orientation (and thus no circular RNA is produced).