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Addgene

pcDNA3.1(+) EPHB4 Sense
(Plasmid #60638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6876
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ephrin type B receptor 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • Entrez Gene
    EPHB4 (a.k.a. CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer PCMV For
  • 3′ sequencing primer bGH PolyA Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid generates the human EPHB4 exon 11/12 circular RNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) EPHB4 Sense was a gift from Jeremy Wilusz (Addgene plasmid # 60638 ; http://n2t.net/addgene:60638 ; RRID:Addgene_60638)

  • For your References section:

    Short intronic repeat sequences facilitate circular RNA production. Liang D, Wilusz JE. Genes Dev. 2014 Oct 3. pii: gad.251926.114. 10.1101/gad.251926.114 PubMed 25281217
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