pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735
(Plasmid #60633)

• Purpose: ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns
• Depositing Lab: Jeremy Wilusz
• Publication: Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.114.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6443
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZKSCAN1
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Entrez Gene: ZKSCAN1 (a.k.a. KOX18, PHZ-37, ZNF139, ZNF36, ZSCAN33)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: PCMV For
• 3′ sequencing primer: bGH PolyA Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression plasmid for the human ZKSCAN1 exon 2/3 circular RNA. This plasmid contains the minimal sufficient introns as diagrammed in Figure 2E of Liang and Wilusz (2014) Genes Dev.