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pJK_kanR_proJK1_PAD
(Plasmid #60630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3582
  • Total vector size (bp) 4026
  • Modifications to backbone
    Deletion of lacI, lacO, and T7 promoter by cloning proJK1 in-between BglI and XbaI.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phenolic acid decarboxylase padC
  • Insert Size (bp)
    486
  • Mutation
    K146Y
  • GenBank ID
    WP_003243190
  • Entrez Gene
    padC (a.k.a. BSU34400)
  • Promoter proJK1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GGTGATTCATTCTGCTAA
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK_kanR_proJK1_PAD was a gift from Timothy Whitehead (Addgene plasmid # 60630 ; http://n2t.net/addgene:60630 ; RRID:Addgene_60630)

  • For your References section:

    Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction. Tomek KJ, Saldarriaga CR, Velasquez FP, Liu T, Hodge DB, Whitehead TA. Biotechnol Bioeng. 2015 Mar;112(3):627-32. doi: 10.1002/bit.25473. Epub 2014 Nov 24. 10.1002/bit.25473 PubMed 25311910
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