pJK_kanR_proJK1_PAD
(Plasmid
#60630)
-
PurposeConstitutively expresses PAD (phenolic acid decarboxylase)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 60630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET-29b(+)
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3582
- Total vector size (bp) 4026
-
Modifications to backboneDeletion of lacI, lacO, and T7 promoter by cloning proJK1 in-between BglI and XbaI.
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePhenolic acid decarboxylase padC
-
Insert Size (bp)486
-
MutationK146Y
-
GenBank IDWP_003243190
-
Entrez GenepadC (a.k.a. BSU34400)
- Promoter proJK1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GGTGATTCATTCTGCTAA
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJK_kanR_proJK1_PAD was a gift from Timothy Whitehead (Addgene plasmid # 60630 ; http://n2t.net/addgene:60630 ; RRID:Addgene_60630) -
For your References section:
Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction. Tomek KJ, Saldarriaga CR, Velasquez FP, Liu T, Hodge DB, Whitehead TA. Biotechnol Bioeng. 2015 Mar;112(3):627-32. doi: 10.1002/bit.25473. Epub 2014 Nov 24. 10.1002/bit.25473 PubMed 25311910