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Addgene

EYFP-NLS-actin-K68/284R
(Plasmid #60616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-actin
  • Species
    H. sapiens (human)
  • Mutation
    K68R, 284R
  • GenBank ID
    NM_001101.3
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Tags / Fusion Proteins
    • EYFP (N terminal on insert)
    • NLS (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Clonetech pEYFP-Actin

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EYFP-NLS-actin-K68/284R was a gift from Primal de Lanerolle (Addgene plasmid # 60616 ; http://n2t.net/addgene:60616 ; RRID:Addgene_60616)

  • For your References section:

    SUMOylation of nuclear actin. Hofmann WA, Arduini A, Nicol SM, Camacho CJ, Lessard JL, Fuller-Pace FV, de Lanerolle P. J Cell Biol. 2009 Jul 27;186(2):193-200. doi: 10.1083/jcb.200905016. 10.1083/jcb.200905016 PubMed 19635839
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