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Addgene

YFP NLS Beta-Actin S14C
(Plasmid #60614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-actin
  • Species
    H. sapiens (human)
  • Mutation
    S14C
  • GenBank ID
    NM_001101.3
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Tags / Fusion Proteins
    • EYFP (N terminal on insert)
    • NLS (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Clonetech pEYFP-Actin
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP NLS Beta-Actin S14C was a gift from Primal de Lanerolle (Addgene plasmid # 60614 ; http://n2t.net/addgene:60614 ; RRID:Addgene_60614)

  • For your References section:

    Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA. Chang L, Godinez WJ, Kim IH, Tektonidis M, de Lanerolle P, Eils R, Rohr K, Knipe DM. Proc Natl Acad Sci U S A. 2011 May 24;108(21):E136-44. doi: 10.1073/pnas.1103411108. Epub 2011 May 9. 10.1073/pnas.1103411108 PubMed 21555562
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