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pENTR-D-TOPO-PAmCherry1-MCS
(Plasmid #60608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 3357
  • Modifications to backbone
    Added flanking MCSs
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmCherry1
  • Alt name
    Photoactivatable mCherry
  • Insert Size (bp)
    708

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was used in Nickerson et al., 2014 (PMID: 24963703) for generation of PAmCherry1 fragments (addgene.org/60545 and addgene.org/60546) for BiFC-PALM.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-TOPO-PAmCherry1-MCS was a gift from Steven Chu & Xiaolin Nan (Addgene plasmid # 60608 ; http://n2t.net/addgene:60608 ; RRID:Addgene_60608)

  • For your References section:

    Single-molecule superresolution imaging allows quantitative analysis of RAF multimer formation and signaling. Nan X, Collisson EA, Lewis S, Huang J, Tamguney TM, Liphardt JT, McCormick F, Gray JW, Chu S. Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18519-24. doi: 10.1073/pnas.1318188110. Epub 2013 Oct 24. 10.1073/pnas.1318188110 PubMed 24158481
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