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Addgene

pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)
(Plasmid #60606)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7272
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PS11-scFvFc-CD28-gp41 (706-713)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1844
  • Entrez Gene
    CD28 (a.k.a. Tp44)
  • Promoter CMV
  • Tag / Fusion Protein
    • C9 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHRev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713) was a gift from Wayne Marasco (Addgene plasmid # 60606 ; http://n2t.net/addgene:60606 ; RRID:Addgene_60606)

  • For your References section:

    Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles. Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Kamau E, Dwyer M, Aird D, Marasco WA. PLoS One. 2008 Sep 11;3(9):e3181. doi: 10.1371/journal.pone.0003181. 10.1371/journal.pone.0003181 PubMed 18784843
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