Skip to main content
Addgene

pHL-EF1a-SphcCas9-iP-A
(Plasmid #60599)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHL-EF1a-GW-iP-A
  • Backbone manufacturer
    Hotta Lab, CiRA
  • Backbone size w/o insert (bp) 6923
  • Total vector size (bp) 9442
  • Modifications to backbone
    Gateway system (attR1/R2) compatible mammalian expression plasmid vector, especially optimized for the use in human iPS cells. Human EF1a promoter allows to express a gene of interest at the high-level in human pluripotent stem cells.
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPR Cas9
  • Alt name
    SphcCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4140
  • Mutation
    Codon usage optimized for human usage

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL-EF1a-SphcCas9-iP-A was a gift from Akitsu Hotta (Addgene plasmid # 60599 ; http://n2t.net/addgene:60599 ; RRID:Addgene_60599)

  • For your References section:

    Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. 10.1016/j.stemcr.2014.10.013 PubMed 25434822
Related items:
Commonly requested with: